Four years after realigning itself as a designer of RFID systems for wireless Internet networks, AeroScout has received a $21 million third round of venture capital that brings its total funding to $41 million.

Two new Silicon Valley firms joined AeroScout’s investor syndicate for the new round, with Menlo Ventures of Menlo Park, Calif., leading the round. Greylock Partners of San Mateo, Calif., was the other first-time investor.

The new money will support continued sales, marketing and development of San Mateo-based AeroScout’s product line, a system of radio frequency identification tags and sensors that operates on networks using the Wi-Fi wireless standard.

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